A nutritious medium used as an alternative to YPD Broth, where a solid base is required.
Glucose is included to promote rapid growth.
Glucose is included to promote rapid growth.
Glucose is included to promote rapid growth.
Pathogenicity of the organism is linked to the production of verocytotoxins (VT1
and VT2), but it should be noted that not all strains of O157:H7 produce verocytotoxins, and that strains from other serovars can be toxin producers (e.g. O26, O111, O113, O145).
O157:H7 has been associated epidemiologically with food poisoning outbreaks involving beefburgers and cold cooked meats. The medium
is a modification of MacConkey Agar No. 3 with the substitution of the fermentable carbohydrate from lactose to sorbitol. O157:H7 is
sorbitol negative and produces translucent colonies whereas most other E. coli strains are sorbitol positive and so produce pink/red
colonies. Selectivity of the medium can be increased by adding Cefixime-Tellurite (C.T.) supplement X161.