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A medium recommended by the Harmonised European Pharmacopoeia for isolation and identification of Salmonella from non-sterile pharmaceutical products.

Conforms to USP/EP/JP performance specification. Originally formulated by Taylor to differentiate enteric pathogens, the agar is widely used as the preferred differential medium for Salmonella spp. The medium is void of peptones but instead uses yeast extract as a carbon, nitrogen and vitamin source and xylose, lactose and sucrose are fermentable carbohydrates. Salmonella are able to ferment xylose to produce acid but not lactose or sucrose. When the xylose is exhausted Salmonella will decarboxylate lysine shifting the pH back to neutral. At near neutral pH, Salmonella can reduce sodium thiosulfate producing hydrogen sulfide which creates a complex with ferric ammonium citrate to produce black or black centred colonies. Other organisms are able decarboxylate lysine but acid production from the fermentation of lactose and sucrose keeps the pH too acidic for H2S production. Selectivity is achieved through the incorporation of sodium deoxycholate and phenol red acts as a pH indicator. According to the Harmonised European Pharmacopoeia, Rappaport Vassiliadis Salmonella Enrichment Broth is used as a selective enrichment broth, with subculture performed onto Xylose Lysine Deoxycholate (XLD) agar.

A medium recommended by the Harmonised European Pharmacopoeia for isolation and identification of bile-tolerant Gram-negative bacteria from non-sterile pharmaceutical products.

Conforms to USP/EP/JP performance specification. Yeast extract provides vitamins and gelatin serves as source of carbon and nitrogen. Glucose is a fermentable carbohydrate and sodium chloride maintains the osmotic balance. Bile salts and crystal violet act as selective agents inhibiting many Gram-positive bacteria. The formulation is a modification of Violet Red Bile Agar by Mossel which substitutes lactose for glucose. Enterobacteriaceae, such as Escherichia coli and Salmonella spp., are able to ferment glucose. This produces acid which results in a pH drop indicated by neutral red resulting in pink colonies. Enough acid production will cause the precipitation of bile salts resulting in a bile precipitate or halo around glucose fermenting bacteria. Non-glucose fermenting bile tolerant bacteria such as Psuedomonas aeruginosa grow but remain colourless with no bile precipitate. According to the Harmonised European Pharmacopoeia, Enterobacteria Enrichment Broth-Mossel is used as a selective enrichment broth, with subculture performed onto Violet Red Bile Glucose Agar (VRBGA).

A medium recommended by the Harmonised European Pharmacopoeia for the enrichment of Candida albicans from non-sterile pharmaceutical samples.

Conforms to USP/EP/JP performance specification. The medium is also used for the cultivation of fungal test strains as described by the Harmonised European Pharmacopoeia. The peptone digests and dextrose provide a nutritious base for luxuriant fungal growth and the acidic pH affords selectivity against bacteria. Due to the high carbohydrate content and low pH this medium is highly sensitive to overheating. According to the Harmonised European Pharmacopoeia, Sabouraud Dextrose Broth is used as an enrichment broth, with subculture performed onto Sabouraud Dextrose Agar.

A medium recommended by the Harmonised European Pharmacopoeia for the isolation and identification of Candida albicans from non-sterile pharmaceutical samples.

Conforms to USP/EP/JP performance specification. The medium is commonly abbreviated to SDA. The medium is also used for the preparation and maintenance of fungal test strains as described by the Harmonised European Pharmacopoeia. The peptone digests and dextrose provide a nutritious base for luxuriant fungal growth and the acidic pH affords selectivity against bacteria. Due to the high carbohydrate content and low pH this medium is highly sensitive to overheating, which will cause a drop in the gel strength. According to the Harmonised European Pharmacopoeia, Sabouraud Dextrose Broth is used as an enrichment broth, with subculture performed onto Sabouraud Dextrose Agar.

A medium recommended by the Harmonised European Pharmacopoeia for the selective enrichment of Clostridia from non-sterile pharmaceutical samples.

Conforms to USP/EP/JP performance specification. The medium is also commonly referred to as Reinforced Clostridial Medium and abbreviated to RCM. Peptone, beef and yeast extract provide a source of nitrogen, essential vitamins and amino acids. Starch aids the detoxification of harmful metabolites and glucose is a fermentable carbohydrate. Sodium chloride provides osmotic balance and sodium acetate acts as a buffer. L-Cysteine act as reducing agents to create an anaerobic environment and maintain a low Eh. This is aided by the low level of agar which reduces the oxygen permeability through the medium. According to the Harmonised European Pharmacopoeia, Reinforced Medium for Clostridia is used as a selective enrichment broth, with subculture performed onto Columbia Agar.

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