MRS Broth is a medium for the cultivation and enumeration of Lactobacillus spp. This product has the same formulation as LAB093 MRS Agar with the omission of agar.
Originally developed in 1960 by de Man, Rogosa & Sharpe, the medium can be used for confirmatory tests on organisms isolated on MRS Agar. The medium can also be used for enumeration by the Miles and Misra technique.
Nutrition is provided by a mixture of carefully selected peptones, glucose, beef & yeast extracts whilst Tween® 80, magnesium and manganese sulphates act as growth stimulants. Selectivity against streptococci & moulds is provided by ammonium citrate and sodium acetate.
Occasionally, sterilisation of this medium at 121oC for 15 minutes, in some autoclaves, may cause the pH to fall outside of the specified pH limits 6.4 +/- 0.2. In these rare cases, adjustment of the medium using acetic acid or sodium hydroxide is recommended.