Immunomagnetic Separation beads for the concentration and isolation of Escherichia coli O45 from food, animal feed, beverages, pharmaceutical or environmental samples.
Captivate™ O45 are magnetisable particles, coated with specific antibody, intended for the concentration and isolation of Escherichia coli O45 from food, animal feed, beverages, pharmaceutical or environmental samples.
Since E. coli O157:H7 was first identified, shiga-toxin producing Escherichia coli (STEC, also referred to as VTEC - verocytotoxin-producing E. coli) of various serotypes have become an increasing concern for public health. Numerous outbreaks have been attributed to STEC serotypes and symptoms may includes bloody or acute diarrhoea, and/or the development in some patients of Haemolytic Ureamic Syndrome (HUS) and Thrombotic Thrombocytopenic Purpura (TTP).
Non-O157 VTEC are associated with the following: fresh meat; ready-to-eat fermented meats (e.g. salami, pepperoni); fresh produce (ready-to-eat pre-cut vegetables and sprouted seeds); raw and low heat-treated milk and derived dairy products; and in the hides and fleeces of cattle, sheep and goats.
The most common serogroup of STEC in Europe and the USA is E. coli O157, however the incidence of other serotypes is increasing. Six major non-O157 STEC serogroups have been identified in the USA in a study conducted at the Centers for Disease Control and Prevention. The study showed that approximately 70% of all incidents of non-O157 between 1983-2002 resulted from O26, O45, O103, O111, O121 and O145 serogroups.
Escherichia coli O45 is not biochemically unique (unlike for example, E. coli O157 or O26 which cannot ferment sorbitol and rhamnose fermentation respectively) and is therefore difficult to isolate from other STEC using conventional culture methods.
Immunomagnetic separation (IMS) is the only process that can be used for the specific isolation of E. coli O45 in which a viable microbiological culture is obtained. Antibodies specific to the O45 serogroup have been attached to the super paramagnetic particles via a covalent coupling process. Captivate™ O45 was developed and tested using faecal samples and pathogenic outbreak strains.
