Immunomagnetic Separation beads for the concentration and isolation of Escherichia coli O111 from food, animal feed, beverages, pharmaceutical or environmental samples.

Captivate™ O111 are magnetisable particles, coated with specific antibody, intended for the concentration and isolation of Escherichia coli O111 from food, animal feed, beverages, pharmaceutical or environmental samples.

Since E. coli O157:H7 was first identified, shiga-toxin producing Escherichia coli (STEC, also referred to as VTEC - verocytotoxin-producing E. coli) of various serotypes have become an increasing concern for public health. Numerous outbreaks have been attributed to STEC serotypes and symptoms may includes bloody or acute diarrhoea, and/or the development in some patients of Haemolytic Ureamic Syndrome (HUS) and Thrombotic Thrombocytopenic Purpura (TTP).

Non-O157 VTEC are associated with the following: fresh meat; ready-to-eat fermented meats (e.g. salami, pepperoni); fresh produce (ready-to eat pre-cut vegetables and sprouted seeds); raw and low heat-treated milk and derived dairy products; and in the hides and fleeces of cattle, sheep and
goats.

Conventional isolation methods commonly use enrichment followed by isolation on culture media. However, E. coli O111 colonies are difficult to differentiate from other serotypes. Immunomagnetic separation offers a way of rapidly separating E. coli O111 from non target bacteria, resulting in
improved rates of isolation.